Dr. Apurva Phale
PhD in Molecular Biophysics
Recent Graduate, National Centre for Biological Sciences (NCBS), TIFR, Bangalore, India
About
My research focuses on understanding conformational exchange in proteins using solution NMR spectroscopy and computational methods. I develop and apply relaxation dispersion experiments, including enhanced CPMG techniques, to characterize protein dynamics across timescales ranging from sub-milliseconds to seconds. By integrating these NMR approaches with mass spectrometry and structural biology, I investigate how missense mutations affect protein stability, structure, and function in disease-relevant contexts such as DNA damage repair.
Seeking Postdoctoral Opportunities — I recently graduated with a PhD in Molecular Biophysics. I'm interested in positions in protein dynamics, molecular biophysics, and computational biology.
Research Interests
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Structural Biology & NMR Spectroscopy
Protein structure determination and development of NMR methodologies including eCPMG relaxation dispersion experiments
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Protein Dynamics & Conformational Exchange
Characterizing conformational exchange processes across timescales ranging from microseconds to seconds
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Mass Spectrometry & Proteomics
Studying protein complexes, post-translational modifications, and protein-protein interactions
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Structure-Function Relationships
Investigating how missense mutations affect protein stability, structure, and function in disease-relevant contexts
Technical Skills
Structural Biology
- Solution NMR Spectroscopy
- Protein Structure Determination
- Relaxation Dispersion Experiments (CPMG, eCPMG)
- Molecular Dynamics Simulations
Biochemistry & Biophysics
- Mass Spectrometry
- Protein Expression & Purification
- Biophysical Characterization
- Isotope Labeling
Computational
- NMR Data Analysis (NMRPipe, CCPN)
- Structural Modeling & Refinement
- Python & Data Analysis
- Molecular Visualization (PyMOL, UCSF Chimera)
Specialized Techniques
- Protein Dynamics Analysis
- Mutation Impact Assessment
- Structural Database Deposition (PDB, BMRB)
- Scientific Writing & Communication